Karen Zito

Karen Zito's picture
Associate Professor

PhD

(530) 752-7832
kzito@ucdavis.edu

 

 

 

 

 

Mechanisms of experience-dependent synaptic plasticity 

The goal of our research is to understand at the cellular and molecular level how synaptic connections form during development, how they are modified by experience, and how they are altered in disease. Most of the excitatory synaptic connections in the cortex occur on dendritic spines, tiny protrusions that extend from the dendritic membrane. Dendritic spines are highly dynamic during development both in vitro and in vivo; periods of high motility coincide with synapse formation. Spine motility, driven by actin dynamics, is thought to allow the postsynaptic neuron to explore and sample presynaptic partners. In addition, alterations of spine dynamics and stability have been observed in response to sensory experience, leading to the hypothesis that these anatomical changes underlie the adaptive remodeling of cortical circuits.

The identification and characterization of the molecules and mechanisms that control spine morphogenesis will be a crucial step toward understanding the formation and plasticity of cortical circuits. Our approach to this problem combines time-lapse imaging to observe nascent spine formation, and fluorescence recovery after photobleaching (FRAP) to measure protein dynamics, with molecular manipulations of synaptic proteins to decipher their roles in the growth of dendritic spines and synapses. In addition, we use physiological measurements in combination with two-photon uncaging of glutamate to examine the function of nascent synapses at the single synapse level.


Teaching Interests: Developmental Neurobiology; Cell Biology of the Neuron; Microscopy

Selected Publications: 

Oh WC, Hill TC, Zito K (2013) Synapse-specific and size-dependent mechanisms of spine structural plasticity accompanying synaptic weakening. PNAS 110(4):E305-12.

Hill TC, Zito K (2013) LTP-induced long-term stabilization of individual nascent dendritic spines. J Neurosci 33:678-86.

Hamilton, AM, Oh WC, Vega-Ramirez H, Stein IS, Hell JW, Patrick GN, Zito K (2012) Activity-dependent growth of new dendritic spines is regulated by the proteasome. Neuron 74:1023-30

Woods G, Oh WC, Boudewyn LC, Mikula SK, Zito K (2011) Loss of PSD-95 is not a prerequisite for spine retraction. J Neurosci 31: 12129-38.

Zito K, Scheuss V, Knott G, Hill T, Svoboda K (2009) Rapid development of glutamatergic synapses on nascent spines, Neuron 61: 247-258.

Woods G and Zito K (2008) Preparation of gene gun bullets and biolistic transfection of neurons in slice culture. JoVE 12, http://www.jove.com/index/Details.stp?ID=675, doi: 10.3791/675.

Zito K and Scheuss V (2007) Glutamate: NMDA receptor function, physiological modulation. The New Encyclopedia of Neuroscience, edited by Larry Squire, Elsevier Press.

Zito K, Knott G, Shepherd GMG, Shenolikar S, Svoboda K (2004) Induction of spine growth and synapse formation by regulation of the spine actin cytoskeleton. Neuron 44: 321-334.

Zito K (2003) The flip side of synapse elimination. Neuron 37:1-2.

Zito K and Svoboda K (2002) Activity-dependent synaptogenesis in the adult mammalian cortex. Neuron 35: 1015-1017.

Zito K and Murthy V (2002) Dendritic spines. Current Biology 12:R5.

Other Affiliations: 

Biochemistry and Molecular Biology Cell and Developmental Biology Neuroscience

Current Students: 
Jason Lambert
Postdocs and Research Personnel: 
Laxmi Parajuli Ivar Stein
Staff Members: 
Julie Culp, SRA II, (530) 752-7839, jculp(at)ucdavis(dot)edu

Current Students

Name Phone Email
Jason Lambert

Postdocs and Research Personnel

Name Current Position Title Phone Email
Laxmi Parajuli
Ivar Stein

Staff Members

Name Title Phone Email
Julie Culp, SRA II, (530) 752-7839, jculp(at)ucdavis(dot)edu