Andrew Ishida, Ph.D.

 Andrew  Ishida, Ph.D.

Position

  • Professor
  • Neurobiology, Physiology and Behavior

Modulation of Retinal Ganglion Cell Spiking

Research Summary

Vertebrate retinae use fast neurotransmitters to signal moment-to-moment changes in the distribution of incident light. Retinae contain an additional set of neurotransmitters which operate on slower time scales to modulate signal generation and transmission. One of these slowly acting neurotransmitters - dopamine - has been found to regulate light responses, chemical synapses, electrical coupling, and extrasynaptic currents of cells at every level of the retina. Because endogenous dopamine release is increased by illumination, these changes help the retina function as the "front end" of the visual system during daylight. Our laboratory studies how dopamine modulates action potentials and voltage-gated ion currents in adult mammalian retinal ganglion cells. We are identifying effects of dopamine and related ligands on ganglion cell excitability, digital and analog properties of spikes, currents activated by either depolarization or hyperpolarization, and signaling cascade components. We are pursuing these projects by use of patch-clamp recording, fast voltage-clamp, multielectrode array recording, immunohistochemistry, immunoprecipitation, calcium imaging, primary cell culture, and organotypic culture. 

Courses Taught:

NSC 261A Topics in Vision

NPB 101 Systemic Physiology 

Select Publications

Fasoli A, Dang JA, Johnson JS, Gouw AH, Fogli Iseppe A, Ishida AT (2017). Somatic and neuritic spines on tyrosine hydroxylase-immunopositive cells of rat retina. Journal of Comparative Neurology 525: 1707-1730.

Stradleigh TW, Greenberg KP, Partida GJ, Pham A, Ishida AT (2015). Moniliform deformation of retinal ganglion cells by formaldehyde-based fixatives. Journal of Comparative Neurology 523: 545-564. (Cover article)

Stradleigh TW, Ishida AT (2015). Fixation strategies for retinal immunohistochemistry. Progress in Retinal and Eye Research 48: 181-202.

Ogata G, Stradleigh TW, Partida GJ, Ishida AT (2012) Dopamine and full-field illumination activate D1 and D2-D5-type receptors in adult rat retinal ganglion cells. Journal of Comparative Neurology 520: 4032-4049.

Partida GJ, Stradleigh TW, Ogata G, Godzdanker I, Ishida AT (2012). Thy1 associates with the cation channel subunit HCN4 in adult rat retina. Investigative Ophthalmology and Visual Science 53: 1696-1703.

Stradleigh TW, Ogata G, Partida GJ, Oi H, Greenberg KP, Krempely KS, Ishida AT (2011). Colocalization of HCN channel subunits in rat retinal ganglion cells. Journal of Comparative Neurology 519: 2546-2573.

Hayashida Y, Varela Rodríguez C, Ogata G, Partida GJ, Oi H, Stradleigh TW, Lee SC, Felipe Colado A, Ishida AT (2009). Inhibition of adult rat retinal ganglion cells by D1-type dopamine receptor activation. Journal of Neuroscience 29: 15001–15016.

Oi H, Partida GJ, Lee SC, Ishida AT (2008). HCN4-like immunoreactivity in rat retinal ganglion cells. Visual Neuroscience 25: 95-102.

Lee SC, Ishida AT (2007). I(h) without K(ir) in adult rat retinal ganglion cells. Journal of Neurophysiology 97: 3790-3799.

Partida GJ, Lee SC, Haft-Candell L, Nichols GS, Ishida AT (2004). DARPP-32-like immunoreactivity in AII amacrine cells of rat retina. Journal of Comparative Neurology 480: 251-263

Hayashida Y, Ishida AT (2004). Dopamine receptor activation can reduce voltage-gated Na+ current by modulating both entry into and recovery from inactivation. Journal of Neurophysiology 92: 3134-3141.

Lee SC, Y Hayashida, Ishida AT (2003). Availability of low-threshold Ca2+ current in retinal ganglion cells. Journal of Neurophysiology 90: 3888-3901.

Vaquero CF, A Pignatelli, GJ Partida, Ishida AT (2001). A dopamine- and protein kinase A-dependent mechanism for network adaptation in retinal ganglion cells. Journal of Neuroscience 21:8624-8635.

Affiliations

Molecular Cellular and Integrative Physiology