Laura Borodinsky, Ph.D.

 Laura  Borodinsky, Ph.D.

Position

  • Assistant Professor
  • Physiology and Membrane Biology (Shriners Hospital for Children)

Developmental Neuroscience

Research Summary

We are interested in investigating the cellular and molecular mechanisms by which activity controls the development of the nervous system. Different forms of activity are present at early stages of development, substantially before synapse formation, suggesting that neuronal activity participates in early steps of differentiation. Neurotransmitter and neurotransmitter receptor specification, axonal pathfinding, synapse formation and function are some of the aspects of nervous system development we are currently studying. Our lab works on Xenopus laevis as a model system using a combination of methodologies including confocal microscopy, immunostaining, molecular biology, pharmacology, and calcium and chloride imaging.

Select Publications

Borodinsky LN, Spitzer NC (2007) Activity-Dependent Neurotransmitter-Receptor Matching at the Neuromuscular Junction. PNAS, 104: 335-340.

Borodinsky LN, Spitzer NC (2006) Second messenger pas de deux: The coordinated dance between calcium and cAMP. Sci. STKE, 2006: pe22.

Spitzer NC, Borodinsky LN, Root CM (2005) Homeostatic activity-dependent paradigm for neurotransmitter specification. Cell Calcium, 37: 417-423.

Spitzer NC, Borodinsky LN, Root CM. (2005) Imaging calcium transients in developing Xenopus spinal neurons. In "Imaging in neuroscience and development: A laboratory manual". Yuste R and Konnerth A, Eds, Cold Spring Harbor Laboratory Press.

Spitzer NC, Root CM, Borodinsky LN (2004) Orchestrating neuronal differentiation: patterns of Ca2+ spikes specify transmitter choice. Trends Neurosci 27: 415-421.

Borodinsky LN, Root CM, Cronin J, Sann SB, Gu X, Spitzer NC. (2004) Activity-dependent homeostatic specification of transmitter expression in embryonic neurons. Nature 429: 523-530.

Borodinsky LN, OLeary D, Neale JH, Vicini S, Coso OA, Fiszman ML (2003) GABA-induced neurite outgrowth of cerebellar granule cells is mediated by GABAA receptor activation, calcium influx and CaMKII and MEK1 pathways. J Neurochem 84: 1411-1420.

Borodinsky LN, Coso OA, Fiszman ML (2002) Contribution of Ca2+-calmodulin-dependent protein kinase II and mitogen-activated protein kinase kinase on neural activity-induced neurite outgrowth and survival of cerebellar granule cells. J Neurochem 80: 1062-1070.

Borodinsky LN, Fiszman ML (2001) A single cell model to study changes in neuronal fractal dimension. Methods 24: 341-345.

Fiszman ML, Borodinsky LN, Neale JH (1999) GABA induces proliferation of immature cerebellar granule cells grown in vitro. Dev Brain Res 115: 1-8.

Borodinsky LN, Fiszman ML (1998) Extracellular potassium concentration regulates proliferation of immature cerebellar granule cells. Dev Brain Res 107: 43-48.

Borodinsky LN, Pesce G, Pomata PE, Fiszman ML (1997) Neurosteroid modulation of GABAA receptors in the developing rat brain cortex. Neurochem Int 31: 313-317.

Affiliations

Physiology and Membrane Biology

Institute for Pediatric Regenerative Medicine

Center for Neuroscience

Cell and Developmental Biology

Molecular Cellular and Integrative Physiology

Neuroscience